Purification of cysteine proteinases from trichomonads using bacitracin-sepharose

نویسندگان
چکیده

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Purification and characterisation of cysteine proteinases from human osteoclastomas.

The process of normal bone remodeling requires the continual resorption and replacement of bone mineral and bone matrix proteins. Excessive bone resorption is a feature of a number of pathological conditions. These include osteoporosis, rheumatoid arthritis and Paget's disease [l]. Bone resorption is accomplished by the osteoclast. Osteoclasts attached to bone are highly polarised cells [2]. Th...

متن کامل

The use of phenyl-Sepharose for the affinity purification of proteinases.

Phenyl-Sepharose is most often used as an adsorbent for hydrophobic interaction chromatography (HIC). We report on its effective use for the affinity purification of some extracellular thermostable proteinases from bacterial sources. Proteinases belonging to the serine, aspartate and metallo mechanistic classes were effectively retained by the media. Purification factors in the range of 2.9-60 ...

متن کامل

Isolation, Purification and Characterization of Bacitracin from Bacillus

Bacterial cultures of bacillus species capable of producing bacitracin were isolated from local habitats (garden soil, timber yard soil and sea water) and screened for the production of bacitracin on nutrient media against test organisms viz: Micrococcus luteus,(ATCC 9341) & Staphylococcus aureus (ATCC 13565).Optimization of culture conditions and media composition for bacitracin production was...

متن کامل

Cysteine proteinases and the pathogenesis of amebiasis.

Amebiasis is a major cause of morbidity and mortality throughout the tropical world. Entamoeba histolytica is now recognized as a separate species from the morphologically identical E. dispar, which cannot invade. Cysteine proteinases are a key virulence factor of E. histolytica and play a role in intestinal invasion by degrading the extracellular matrix and circumventing the host immune respon...

متن کامل

Labelling of cysteine proteinases in purified lysosomes.

NADH could not be freeze-stored at -25°C in 50 mMsodium phosphate buffer (pH range 6-8 20°C) without serious loss of the nucleotide; however, no losses were seen upon freeze storage in 50 mM-Hepes buffer (pH range 7-8, 20°C). The rate of NADH degradation was greater (approx. 2-fold) in 50 mMthan in 5 mM-sodium phosphate buffer, although NADH values at both buffer concentrations were similar (5%...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: FEMS Microbiology Letters

سال: 1993

ISSN: 0378-1097,1574-6968

DOI: 10.1111/j.1574-6968.1993.tb06304.x